Menu

Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Advanced Light Microscopy

Nikon Confocal A1RHD microscope

Fully-automated confocal imaging system equipped with both a high-resolution galvano scanner as well as a high-speed (15 fps) 1024 x 1024 resonant scanner capable of capturing high-quality confocal images of tissues, cells and molecular events at high speed and enhanced sensitivity. This system is also equipped with a spectral detector with a 10nm range resolution.

Key features:

Available laser lines: 405 nm, 488 nm, 561 nm, and 640 nm

Detectors: 2 GaAsP PMT (for 488 and 561 lines), 2 PMT (for 405 and 640 lines) and 1 GaAsP PMT for spectral detection

Piezo Z-drive (NanoDrive, MadCity labs) for fast Z-stacks

Software: NIS-Elements

 

Nikon Confocal A1+ microscope

Fully-automated confocal imaging system equipped with a high-resolution galvano scanner capable of capturing high-quality confocal images of tissues, cells and molecular events at enhanced sensitivity.

Available laser lines: 405 nm, 488 nm, 561 nm, and 640 nm

Detectors: 2 GaAsP PMT (for 488 and 561 lines), 2 PMT (for 405 and 640 lines)

Software: NIS-Elements

To find out more about the confocal technique go to: http://www.microscopyu.com/articles/confocal/confocalintrobasics.html

 

Nikon TIRF/STORM microscope

Total Internal Reflection Fluorescence (TIRF) microscopy gives superior signal to noise images compared to conventional wide-field fluorescence. The system is equipped with an Andor Ultra 897 EMCCD camera and an Andor Zyla 4.2 sCMOS camera (owned by Pontus Nordenfelts group), which enables imaging at very high speeds and sensitivity. The high speeds of the cameras allow for tracking of low-light dynamic events within a live cell.

The STochastic Optical Reconstruction Microscope (STORM) is a super resolution technique that uses technology licensed from Harvard University. N-STORM provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes. The LBIC STORM is equipped with 2 high-powered lasers (561 and 647) that enable 2 color direct STORM. Compared to conventional wide-field and confocal imaging techniques STORM requires specific sample preparation and careful optimization as well as a data reconstruction process.

Available laser lines: 405 nm, 488 nm, 561 nm (high-powered), and 647 nm (high powered)

Cameras: Andor iXon 897 EMCCD and Andor Zyla 4.2 sCMOS

Incubator: Stage top incubator with temperature control.

Software: NIS-Elements

To find out more about the TIRF technique go to http://www.microscopyu.com/articles/fluorescence/tirf/tirfintro.html

To find out more about the STORM technique go to: http://www.microscopyu.com/print/articles/superresolution/stormintro-print.html

 

Contact Sebastian Wasserstrom via email at Sebastian [dot] Wasserstrom [at] med [dot] lu [dot] se for project discussions.

Page Manager:

Lund university Bioimaging Centre
Klinikgatan 32
221 84 Lund
Phone: 046-222 00 00 (switchboard)